Abstract

Studies of chromosomes of Cyrtodactylus jarujini Ulber, 1993 and C. doisuthep Kunya et al., 2014 to compare microsatellite and TTAGGG sequences by classical and molecular techniques were conducted in Thailand. Karyological typing from a conventional staining technique of C. jarujini and C. doisuthep showed diploid chromosome numbers of 40 and 34 while the Fundamental Numbers (NF) were 56 in both species. In addition, we created the chromosome formula of the chromosomes of C. jarujini showing that 2n (40) = Lsm1 + Lsm2 + Lt3 + Mm1 + Mt4 + Sm2 + Sa2 + St5 while that of C. doisuthep was 2n (34) = Lsm3 + Lm2 + Lt3 + Mm1 + Mt2 + Sm4 + Sa1 + St1. Ag-NOR staining revealed NOR-bearing chromosomes in chromosome pairs 13 and 14 in C. jarujini, and in chromosome pairs 9 and 13 in C. doisuthep. This molecular study used the FISH technique, as well as microsatellite probes including (A)20, (TA)15, (CGG)10, (CGG)10, (GAA)10, (TA)15 and TTAGGG repeats. The signals showed that the different patterns in each chromosome of the Gekkonids depended on probe types. TTAGGG repeats showed high distribution on centromere and telomere regions, while (A)20, (TA)15, (CGG)10, (CGG)10, (GAA)10 and (TA)15 bearing dispersed over the whole genomes including chromosomes and some had strong signals on only a pair of homologous chromosomes. These results suggest that the genetic linkages have been highly differentiated between the two species.

Addeddate

2025-03-16 10:38:47

Bhl_virtual_titleid

210879

Bhl_virtual_volume

v.15:no.1 (2021)

Call number

10_3897_CompCytogen_v15_i1_58208

Call-number

10_3897_CompCytogen_v15_i1_58208

Foldoutcount

0

Genre

article

Identifier

cytogeneticchar15thon

Identifier-ark

ark:/13960/s20k1j1040g

Identifier-bib

10_3897_CompCytogen_v15_i1_58208

Identifier-doi

10.3897/CompCytogen.v15.i1.58208

Issue

1

Ocr

tesseract 5.3.0-6-g76ae

Ocr_detected_lang

en

Ocr_detected_lang_conf

1.0000

Ocr_detected_script

Latin

Ocr_detected_script_conf

1.0000

Ocr_module_version

0.0.21

Ocr_parameters

-l eng

Page_number_confidence

78

Page_number_module_version

1.0.5

Page_range

41-52

Pages

12

Pdf_degraded

invalid-jp2-headers

Pdf_module_version

0.0.25

Possible copyright status

In copyright. Digitized with the permission of the rights holder.

Ppi

300

Source

Comparative Cytogenetics 15(1)

Year

2021

Show More

Show Less